In Vitro ADME and Pharmacokinetics

The Moulder Center for Drug Discovery Research is equiped to provide a wide range of in vitro ADME/PK studies, drug metabolism studies and in vivo Pharmacokinetics (PK) studies in support of drug discovery programs.


Available studies include:

In Vitro Assays

  • Microsomal Stability – Human and preclinical species.
  • Hepatocyte Stability – Determine direct conjugation or metabolism by aldehyde oxidase.
  • Unbound fraction assays – Equilibrium dialysis and LC/MSMS analysis and interpretation.
  • CYP inhibition assays - CYP3A4, CYP2D6 and CYP2C9.
  • Permeability assays – CACO-2 and MDCK for correlation with absorption and BBB.
  • Metabolite ID – Tissue preparations, expressed enzymes and LC/MSMS identification.

In Vivo Assays

  • Preclinical Pharmacokinetics – Mouse and rat studies (IV and oral) to determine
    • Volume of distribution
    • Clearance
    • Half-life
    • Bioavailability
  • CNS Penetration - Mouse and rat with SC, IP, or IV administration. 

For additional information on high throughput in vitro ADME and physicochemical screening contact:

Wayne Childers, Ph.D.
Associate Director of the Moulder Center for Drug Discovery Research
Associate Professor, Medicinal Chemistry
215-707-1079
wayne.childers@temple.edu

John C. Gordon, Ph.D.
Manager, Screening Center
Moulder Center for Drug Discovery Research
215-707-4654
john.gordon0001@temple.edu

For additional information on In vitro and in vivo PK studies contact:

Kenneth Korzekwa, Ph.D.
Associate Professor
Department of Pharmaceutical Sciences
215-707-7892
kenneth.korzekwa@temple.edu

Swati Nagar, Ph.D.
Associate Professor
Department of Pharmaceutical Sciences
215-707-9110
swati.nagar@temple.edu